NCLEX: Antifungal Drugs

Infectious diseases caused by fungi are called mycoses, and they are often chronic in nature. Mycotic infections may be superficial and involve only the skin (cutaneous mycoses extending into the epidermis), while others may penetrate the skin, causing subcutaneous or systemic infections. The characteristics of fungi are so unique and diverse that they are classified in their own kingdom. Unlike bacteria, fungi are eukaryotic, with rigid cell walls composed largely of chitin rather than peptidoglycan (a characteristic component of most bacterial cell walls). In addition, the fungal cell membrane contains ergosterol rather than the cholesterol found in mammalian membranes. These structural characteristics are useful in targeting chemotherapeutic agents against fungal infections. Fungal infections are generally resistant to antibiotics, and, conversely, bacteria are resistant to antifungal agents. The incidence of fungal infections such as candidemia has been on the rise for the last few decades. This is attributed to an increased number of patients with chronic immune suppression due to organ transplantation, cancer chemotherapy, or infection with human immunodeficiency virus (HIV). During this same period, new therapeutic options have become available for the treatment of fungal infections.

Antifungal Drugs

Antifungal Drugs

Antifungal Drugs

Antifungal Drugs: DRUGS FOR SUBCUTANEOUS AND SYSTEMIC MYCOTIC INFECTIONS

Focus topic: Antifungal Drugs

A. Amphotericin B

Amphotericin [am-foe-TER-i-sin] B is a naturally occurring polyene antifungal produced by Streptomyces nodosus. In spite of its toxic potential, amphotericin B remains the drug of choice for the treatment of several life-threatening mycoses.

  • Mechanism of action: Amphotericin B binds to ergosterol in the plasma membranes of sensitive fungal cells. There, it forms pores (channels) that require hydrophobic interactions between the lipophilic segment of the polyene antifungal and the sterol. The pores disrupt membrane function, allowing electrolytes (particularly potassium) and small molecules to leak from the cell, resulting in cell death.
  • Antifungal spectrum: Amphotericin B is either fungicidal or fungistatic, depending on the organism and the concentration of the drug. It is effective against a wide range of fungi, including Candida albicans, Histoplasma capsulatum, Cryptococcus neoformans, Coccidioides immitis, Blastomyces dermatitidis, and many strains of Aspergillus. [Note: Amphotericin B is also used in the treatment of the protozoal infection leishmaniasis.]
  • Resistance: Fungal resistance, although infrequent, is associated with decreased ergosterol content of the fungal membrane.
  • Pharmacokinetics: Amphotericin B is administered by slow, intravenous (IV) infusion. Amphotericin B is insoluble in water and must be coformulated with either sodium deoxycholate (conventional) or a variety of artificial lipids to form liposomes. The liposomal preparations have the primary advantage of reduced renal and infusion toxicity. However, due to high cost, liposomal preparations are reserved mainly as salvage therapy for patients who cannot tolerate conventional amphotericin B. Amphotericin B is extensively bound to plasma proteins and is distributed throughout the body. Inflammation favors penetration into various body fluids, but little of the drug is found in the CSF, vitreous humor, or amniotic fluid. However, amphotericin B does cross the placenta. Low levels of the drug and its metabolites appear in the urine over a long period of time, and some are also eliminated via the bile. Dosage adjustment is not required in patients with hepatic dysfunction, but when conventional amphotericin B causes renal dysfunction, the total daily dose is decreased by 50%.
  • Adverse effects: Amphotericin B has a low therapeutic index. The total adult daily dose of the conventional formulation should not exceed 1.5 mg/kg/d, whereas lipid formulations have been given safely in doses up to 10 mg/kg/d.

a. Fever and chills: These occur most commonly 1 to 3 hours after starting the IV administration but usually subside with repeated administration of the drug. Premedication with a corticosteroid or an antipyretic helps to prevent this problem.

b. Renal impairment: Despite the low levels of the drug excreted in the urine, patients may exhibit a decrease in glomerular filtration rate and renal tubular function. Serum creatinine may increase, creatinine clearance can decrease, and potassium and magnesium are lost. Renal function usually returns with discontinuation of the drug, but residual damage is likely at high doses. Azotemia is exacerbated by other nephrotoxic drugs, such as aminoglycosides, cyclosporine, pentamidine, and vancomycin, although adequate hydration can decrease its severity. To minimize nephrotoxicity, sodium loading with infusions of normal saline and the lipid-based amphotericin B products can be used.

c. Hypotension: A shock-like fall in blood pressure accompanied by hypokalemia may occur, requiring potassium supplementation. Care must be exercised in patients taking digoxin and other drugs that can cause potassium fluctuations.

d. Thrombophlebitis: Adding heparin to the infusion can alleviate this problem.

Antifungal Drugs

Antifungal Drugs

Antifungal Drugs

B. Antimetabolite antifungals

Flucytosine [floo-SYE-toe-seen] (5-FC) is a synthetic pyrimidine antimetabolite that is often used in combination with amphotericin B. This combination of drugs is administered for the treatment of systemic mycoses and for meningitis caused by C. neoformans and C. albicans.

  • Mechanism of action: 5-FC enters the fungal cell via a cytosinespecific permease, an enzyme not found in mammalian cells. It is subsequently converted to a series of compounds, including 5-fluorouracil and 5-fluorodeoxyuridine 5′-monophosphate, which disrupt nucleic acid and protein synthesis. [Note: Amphotericin B increases cell permeability, allowing more 5-FC to penetrate the cell and leading to synergistic effects.]
  • Antifungal spectrum: 5-FC is fungistatic. It is effective in combination with itraconazole for treating chromoblastomycosis (causes skin and subcutaneous infections) and in combination with amphotericin B for treating candidiasis and cryptococcosis. Flucytosine can also be used for Candida urinary tract infections when fluconazole is not appropriate; however, resistance can occur with repeated use.
  • Resistance: Resistance due to decreased levels of any of the enzymes in the conversion of 5-FC to 5-fluorouracil (5-FU) and beyond or from increased synthesis of cytosine can develop during therapy. This is the primary reason that 5-FC is not used as a single antimycotic drug. The rate of emergence of resistant fungal cells is lower with a combination of 5-FC plus a second antifungal agent than it is with 5-FC alone.
  • Pharmacokinetics: 5-FC is well absorbed by the oral route. It distributes throughout the body water and penetrates well into the CSF. 5-FU is detectable in patients and is probably the result of metabolism of 5-FC by intestinal bacteria. Excretion of both the parent drug and its minimal metabolites is by glomerular filtration, and the dose must be adjusted in patients with compromised renal function.
  • Adverse effects: 5-FC causes reversible neutropenia, thrombocytopenia, and dose-related bone marrow depression. Caution must be exercised in patients undergoing radiation or chemotherapy with drugs that depress bone marrow. Reversible hepatic dysfunction with elevation of serum transaminases and alkaline phosphatase may occur. Gastrointestinal disturbances (nausea, vomiting, and diarrhea) are common, and severe enterocolitis may also occur.

Antifungal Drugs

C. Azole antifungals

Azole antifungals are made up of two different classes of drugs—imidazoles and triazoles. Although these drugs have similar mechanisms of action and spectra of activity, their pharmacokinetics and therapeutic uses vary significantly. In general, imidazoles are given topically for cutaneous infections, whereas triazoles are given systemically for the treatment or prophylaxis of cutaneous and systemic fungal infections. [Note: Imidazole antifungals are discussed in the section on agents for cutaneous mycotic infections.] The triazole antifungals include fluconazole, itraconazole, posaconazole, and voriconazole.

  • Mechanism of action: Azoles are predominantly fungistatic. They inhibit C-14 α-demethylase (a cytochrome P450 [CYP450] enzyme), thereby blocking the demethylation of lanosterol to ergosterol, the principal sterol of fungal membranes. The inhibition of ergosterol biosynthesis disrupts membrane structure and function, which, in turn, inhibits fungal cell growth.
  • Resistance: Resistance to azole antifungals is becoming a significant clinical problem, particularly with protracted therapy required in immuno-compromised patients, such as those who have advanced HIV infection or bone marrow transplant. Mechanisms of resistance include mutations in the C-14 α-demethylase gene that lead to decreased azole binding. Additionally, some strains of fungi have developed efflux pumps that pump the azole out of the cell.
  • Drug interactions: All azoles inhibit the hepatic CYP450 3A4 isoenzyme to varying degrees. Patients on concomitant medications that are substrates for this isoenzyme may have increased concentrations and risk for toxicity. Several azoles, including itraconazole and voriconazole, are metabolized by CYP450 3A4 and other CYP450 isoenzymes. Therefore, concomitant use of potent CYP450 inhibitors (for example, ritonavir) and inducers (for example, rifampin) can lead to increased adverse effects or clinical failure of these azoles, respectively.
  • Contraindications: Azoles are considered teratogenic, and they should be avoided in pregnancy unless the potential benefit outweighs the risk to the fetus.

Antifungal Drugs

D. Fluconazole

Fluconazole [floo-KON-a-zole] was the first member of the triazole class of antifungal agents. It is the least active of all triazoles, with most of its spectrum limited to yeasts and some dimorphic fungi. It has no role in the treatment of aspergillosis or zygomycosis. It is highly active against Cryptococcus neoformans and certain species of Candida, including C. albicans and C. parapsilosis. Resistance is a concern, however, with other species, including C. krusei and C. glabrata. Fluconazole is used for prophylaxis against invasive fungal infections in recipients of bone marrow transplants. It also is the drug of choice for Cryptococcus neoformans after induction therapy with amphotericin B and flucytosine and is used for the treatment of candidemia and coccidioidomycosis. Fluconazole is effective against most forms of mucocutaneous candidiasis. It is commonly used as a single-dose oral treatment for vulvovaginal candidiasis. Fluconazole is available in oral or IV dosage formulations. It is well absorbed after oral administration and distributes widely to body fluids and tissues. The majority of the drug is excreted unchanged via the urine, and doses must be reduced in patients with renal dysfunction. The most common adverse effects with fluconazole are nausea, vomiting, headache, and skin rashes. Hepatotoxicity can also occur, and the drug should be used with caution in patients with liver dysfunction.

E. Itraconazole

Itraconazole [it-ra-KON-a-zole] is a synthetic triazole that has a broad antifungal spectrum compared to fluconazole. Itraconazole is the drug of choice for the treatment of blastomycosis, sporotrichosis, paracoccidioidomycosis, and histoplasmosis. It is rarely used for treatment of infections due to Candida and Aspergillus species because of the availability of newer and more effective agents. Itraconazole is available in two oral dosage forms, a capsule and an oral solution. The oral capsule should be taken with food, and ideally an acidic beverage, to increase absorption. In contrast, the solution should be taken on an empty stomach, as food decreases the absorption. The drug distributes well in most tissues, including bone and adipose tissues. Itraconazole is extensively metabolized by the liver, and the drug and inactive metabolites are excreted in the feces and urine. Adverse effects include nausea, vomiting, rash (especially in immuno-compromised patients), hypokalemia, hypertension, edema, and headache. Hepatotoxicity can also occur, especially when given with other drugs that affect the liver. Itraconazole has a negative inotropic effect and should be avoided in patients with evidence of ventricular dysfunction, such as heart failure.

F. Posaconazole

Posaconazole [poe-sa-KONE-a-zole], a synthetic triazole, is a broad spectrum antifungal structurally similar to itraconazole. It is available as an oral suspension, oral tablet, or IV formulation. Posaconazole is commonly used for the treatment and prophylaxis of invasive Candida and Aspergillus infections in severely immunocompromised patients. Due to its broad spectrum of activity, posaconazole is also used in the treatment of invasive fungal infections caused by Scedosporium and Zygomycetes. Posaconazole has a low oral bio-availability and should be given with food. Even though posaconazole has a long half-life, the suspension is usually given in divided doses throughout the day due to saturable absorption in the gut, whereas the tablet is given once daily. Unlike other azoles, posaconazole is not metabolized in the liver by CYP450 but is eliminated via glucuronidation. The most common adverse effects include gastrointestinal disturbances (nausea, vomiting, and diarrhea) and headaches. Like other azoles, posaconazole can cause an elevation in serum hepatic transaminases. Drugs that affect the gastric pH (for example, proton pump inhibitors) may decrease the absorption of oral posaconazole and should be avoided if possible. Due to its potent inhibition of CYP3A4, concomitant use of posaconazole with a number of agents (for example, ergot alkaloids, atorvastatin, citalopram, risperidone, pimozide, and quinidine) is contraindicated.

G. Voriconazole

Voriconazole [vor-i-KON-a-zole], a synthetic triazole related to fluconazole, has the advantage of being a broad-spectrum antifungal agent that is available in both IV and oral dosage forms. Voriconazole has replaced amphotericin B as the drug of choice for invasive aspergillosis. It is also approved for treatment of invasive candidiasis, as well as serious infections caused by Scedosporium and Fusarium species. Voriconazole has high oral bioavailability and penetrates into tissues well. Elimination is primarily by metabolism through the CYP450 enzymes. Voriconazole displays nonlinear kinetics, which can be affected by drug interactions and pharmacogenetic variability, particularly CYP450 2C19 polymorphisms. Adverse effects are similar to those of the other azoles; however, high trough concentrations are associated with visual and auditory hallucinations and an increased incidence of hepatotoxicity. Voriconazole is not only a substrate but also an inhibitor of CYP2C19, 2C9, and 3A4 isoenzymes. Inhibitors and inducers of these enzymes may impact levels of voriconazole, leading to toxicity or clinical failure, respectively. In addition, drugs that are substrates of these enzymes are impacted by voriconazole. Due to significant interactions, use of voriconazole is contraindicated with many drugs (for example, rifampin, rifabutin, carbamazepine, and the herb St. John’s wort).

Antifungal Drugs

Antifungal Drugs

Antifungal Drugs

H. Echinocandins

Echinocandins interfere with the synthesis of the fungal cell wall by inhibiting the synthesis of β(1,3)-d-glucan, leading to lysis and cell death. Caspofungin, micafungin, and anidulafungin are available for IV administration once daily. Micafungin is the only echinocandin that does not require a loading dose. The echinocandins have potent activity against Aspergillus and most Candida species, including those species resistant to azoles. However, they have minimal activity against other fungi. All three agents are well tolerated, with the most common adverse effects being fever, rash, nausea, and phlebitis at the infusion site. They can also cause a histamine-like reaction (flushing) when infused too rapidly.

  • Caspofungin: Caspofungin [kas-poh-FUN-jin] was the first member of the echinocandin class of antifungal drugs. Caspofungin is a first-line option for patients with invasive candidiasis, including candidemia, and a second-line option for invasive aspergillosis in patients who have failed or cannot tolerate amphotericin B or an azole. The dose of caspofungin does not need to be adjusted in renal impairment, but adjustment is warranted with moderate hepatic dysfunction. Concomitant administration of caspofungin with certain CYP450 enzyme inducers (for example, rifampin) may require an increase in the daily dose. Caspofungin should not be co-administered with cyclosporine due to a high incidence of elevated hepatic transaminases with concurrent use.
  • Micafungin and anidulafungin: Micafungin [mi-ka-FUN-jin] and anidulafungin [ay-nid-yoo-la-FUN-jin] are newer members of the echinocandin class of antifungal drugs. Micafungin and anidulafungin are first-line options for the treatment of invasive candidiasis, including candidemia. Micafungin is also indicated for the prophylaxis of invasive Candida infections in patients who are undergoing hematopoietic stem cell transplantation. Micafungin and anidulafungin do not need to be adjusted in renal impairment or mild to moderate hepatic dysfunction. Anidulafungin can be administered in severe hepatic dysfunction, but micafungin has not been studied in this condition. These agents are not substrates for CYP450 enzymes and do not have any associated drug interactions.

Antifungal Drugs: DRUGS FOR CUTANEOUS MYCOTIC INFECTIONS

Focus topic: Antifungal Drugs

Mold-like fungi that cause cutaneous infections are called dermatophytes or tinea. Tinea infections are classified by the affected site (for example, tinea pedis, which refers to an infection of the feet). Common dermatomycoses, such as tinea infections that appear as rings or round red patches with clear centers, are often referred to as “ringworm.” This is a misnomer because fungi rather than worms cause the disease. The three different fungi that cause the majority of cutaneous infections are Trichophyton, Microsporum, and Epidermophyton.

A. Squalene epoxidase inhibitors

These agents act by inhibiting squalene epoxidase, thereby blocking the biosynthesis of ergosterol, an essential component of the fungal cell membrane. Accumulation of toxic amounts of squalene results in increased membrane permeability and death of the fungal cell.

Antifungal Drugs

1. Terbinafine: Oral terbinafine [TER-bin-a-feen] is the drug of choice for treating dermatophyte onychomycoses (fungal infections of nails). It is better tolerated, requires a shorter duration of therapy, and is more effective than either itraconazole or griseofulvin. Therapy is prolonged (usually about 3 months) but considerably shorter than that with griseofulvin. Oral terbinafine may also be used for tinea capitis (infection of the scalp). [Note: Oral antifungal therapy (griseofulvin, terbinafine, itraconazole) is needed for tinea capitis. Topical antifungals are ineffective.] Topical terbinafine (1% cream, gel or solution) is used to treat tinea pedis, tinea corporis (ringworm), and tinea cruris (infection of the groin). Duration of treatment is usually 1 week.

  • Antifungal spectrum: Terbinafine is active against Trichophyton. It may also be effective against Candida, Epidermophyton, and Scopulariopsis, but the efficacy in treating clinical infections due to these pathogens has not been established.
  • Pharmacokinetics: Terbinafine is available for oral and topical administration, although its bioavailability is only 40% due to first-pass metabolism. Terbinafine is highly protein bound and is deposited in the skin, nails, and adipose tissue. Terbinafine accumulates in breast milk and should not be given to nursing mothers. A prolonged terminal half-life of 200 to 400 hours may reflect the slow release from these tissues. Oral terbinafine is extensively metabolized by several CYP450 isoenzymes and is excreted mainly via the urine. The drug should be avoided in patients with moderate to severe renal impairment or hepatic dysfunction.
  • Adverse effects: Common adverse effects of terbinafine include gastrointestinal disturbances (diarrhea, dyspepsia, and nausea), headache, and rash. Taste and visual disturbances have been reported, as well as transient elevations in serum hepatic transaminases. Terbinafine is an inhibitor of the CYP450 2D6 isoenzyme, and concomitant use with substrates of that isoenzyme may result in an increased risk of adverse effects with those agents.

Antifungal Drugs

2. Naftifine: Naftifine [NAF-ti-feen] is active against Trichophyton, Microsporum, and Epidermophyton. Naftifine 1% cream and gel are used for topical treatment of tinea corporis, tinea cruris, and tinea pedis. Duration of treatment is usually 2 weeks.

3. Butenafine: Butenafine [byoo-TEN-a-feen] is active against Trichophyton rubrum, Epidermophyton, and Malassezia. Like naftifine, butenafine 1% cream is used for topical treatment of tinea infections.

B. Griseofulvin

Griseofulvin [gris-ee-oh-FUL-vin] causes disruption of the mitotic spindle and inhibition of fungal mitosis. It has been largely replaced by oral terbinafine for the treatment of onychomycosis, although it is still used for dermatophytosis of the scalp and hair. Griseofulvin is fungistatic and requires a long duration of treatment (for example, 6 to 12 months for onychomycosis). Duration of therapy is dependent on the rate of replacement of healthy skin and nails. Ultrafine crystalline preparations are absorbed adequately from the gastrointestinal tract, and absorption is enhanced by high-fat meals. The drug concentrates in skin, hair, nails, and adipose tissue. Griseofulvin induces hepatic CYP450 activity, which increases the rate of metabolism of a number of drugs, including anticoagulants. The use of griseofulvin is contraindicated in pregnancy and patients with porphyria.

Antifungal Drugs

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C. Nystatin

Nystatin [nye-STAT-in] is a polyene antifungal, and its structure, chemistry, mechanism of action, and resistance profile resemble those of amphotericin B. It is used for the treatment of cutaneous and oral Candida infections. The drug is negligibly absorbed from the gastrointestinal tract, and it is not used parenterally due to systemic toxicity (acute infusion-related adverse effects and nephrotoxicity). It is administered as an oral agent (“swish and swallow” or “swish and spit”) for the treatment of oropharyngeal candidiasis (thrush), intravaginally for vulvovaginal candidiasis, or topically for cutaneous candidiasis. Adverse effects are rare after oral administration, but nausea and vomiting occasionally occur. Topical and vaginal forms may cause skin irritation.

D. Imidazoles

Imidazoles are azole derivatives, which currently include butoconazole [byoo-toe-KON-a-zole], clotrimazole [kloe-TRIM-a-zole], econazole [e-KONE-a-zole], ketoconazole [kee-toe-KON-a-zole], miconazole [my-KON-a-zole], oxiconazole [oks-i-KON-a-zole], sertaconazole [serta-KOE-na-zole], sulconazole [sul-KON-a-zole], terconazole [ter-KONa-zole], and tioconazole [tye-oh-KONE-a-zole]. As a class of topical agents, they have a wide range of activity against Epidermophyton, Microsporum, Trichophyton, Candida, and Malassezia, depending on the agent. The topical imidazoles have a variety of uses, including tinea corporis, tinea cruris, tinea pedis, and oropharyngeal and vulvovaginal candidiasis. Topical use is associated with contact dermatitis, vulvar irritation, and edema. Clotrimazole is also available as a troche (lozenge), and miconazole is available as a buccal tablet for the treatment of thrush. Oral ketoconazole has historically been used for the treatment of systemic fungal infections but is rarely used today due to the risk for severe liver injury, adrenal insufficiency, and adverse drug interactions.

E. Ciclopirox

Ciclopirox [sye-kloe-PEER-oks] inhibits the transport of essential elements in the fungal cell, disrupting the synthesis of DNA, RNA, and proteins. Ciclopirox is active against Trichophyton, Epidermophyton, Microsporum, Candida, and Malassezia. It is available in a number of formulations. Ciclopirox 1% shampoo is used for treatment of seborrheic dermatitis. Tinea pedis, tinea corporis, tinea cruris, cutaneous candidiasis, and tinea versicolor may be treated with the 0.77% cream, gel, or suspension.

F. Tolnaftate

Tolnaftate [tole-NAF-tate] distorts the hyphae and stunts mycelial growth in susceptible fungi. Tolnaftate is active against Epidermophyton, Microsporum, and Malassezia furfur. [Note: Tolnaftate is not effective against Candida.] Tolnaftate is used to treat tinea pedis, tinea cruris, and tinea corporis. It is available as a 1% solution, cream, and powder.

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